Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Identifieur interne : 000B76 ( Main/Exploration ); précédent : 000B75; suivant : 000B77Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Auteurs : Jean-Benoît Le Luduec [France] ; Sabine Debeer [France] ; Fabienne Piras [France] ; Christine Andréoni [France] ; Florence Boudet [France] ; Philippe Laurent [France] ; Dominique Kaiserlian [France] ; Bertrand Dubois [France]Source :
- Vaccine [ 1873-2518 ] ; 2016.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques, Animaux, Anticorps antiviraux (sang), Anticorps neutralisants (sang), Cellules dendritiques (immunologie), Cytokines, Femelle, Immunité cellulaire, Immunité humorale, Immunité innée, Injections intradermiques, Maladie d'Aujeszky (), Peau (immunologie), Protéines de l'enveloppe virale (immunologie), Suidae, Vaccination (), Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Vaccins sous-unitaires (administration et posologie), Vaccins sous-unitaires (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux, Vaccins sous-unitaires.
- immunologie : Cellules dendritiques, Peau, Protéines de l'enveloppe virale, Vaccins antiviraux, Vaccins sous-unitaires.
- sang : Anticorps antiviraux, Anticorps neutralisants.
- Adjuvants immunologiques, Animaux, Cytokines, Femelle, Immunité cellulaire, Immunité humorale, Immunité innée, Injections intradermiques, Maladie d'Aujeszky, Suidae, Vaccination.
English descriptors
- KwdEn :
- Adjuvants, Immunologic, Animals, Antibodies, Neutralizing (blood), Antibodies, Viral (blood), Cytokines, Dendritic Cells (immunology), Female, Immunity, Cellular, Immunity, Humoral, Immunity, Innate, Injections, Intradermal, Pseudorabies (prevention & control), Skin (immunology), Swine, Vaccination (methods), Vaccines, Subunit (administration & dosage), Vaccines, Subunit (immunology), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, Subunit, Viral Vaccines.
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- chemical , immunology : Vaccines, Subunit, Viral Envelope Proteins, Viral Vaccines.
- chemical : Adjuvants, Immunologic, Cytokines.
- immunology : Dendritic Cells, Skin.
- methods : Vaccination.
- prevention & control : Pseudorabies.
- Animals, Female, Immunity, Cellular, Immunity, Humoral, Immunity, Innate, Injections, Intradermal, Swine.
Abstract
Intradermal (ID) vaccination constitutes a promising approach to induce anti-infectious immunity. This route of immunization has mostly been studied with influenza split-virion vaccines. However, the efficacy of ID vaccination for sub-unit vaccines in relation to underlying skin innate immunity remains to be explored for wider application in humans. Relevant animal models that more closely mimic human skin immunity than the widely used mouse models are therefore necessary. Here, we show in domestic swine, which shares striking anatomic and functional properties with human skin, that a single ID delivery of pseudorabies virus (PRV) glycoproteins without added adjuvant is sufficient to trigger adaptive cellular and humoral immune responses, and to confer protection from a lethal respiratory infection with PRV. Analysis of early events at the skin injection site revealed up-regulation of pro-inflammatory cytokine and chemokine genes, recruitment of neutrophils and monocytes and accumulation of inflammatory DC. We further show that the sustained induction of pro-inflammatory cytokine genes results from the combined effects of skin puncture, liquid injection in the dermis and viral antigens. These data highlight that immune protection against respiratory infection can be induced by ID vaccination with a subunit vaccine and reveal that adjuvant requirements are circumvented by the mechanical and antigenic stress caused by ID injection, which triggers innate immunity and mobilization of inflammatory DC at the immunization site. ID vaccination with sub-unit vaccines may thus represent a safe and efficient solution for protection against respiratory infections in swine and possibly also in humans, given the similarity of skin structure and function in both species.
DOI: 10.1016/j.vaccine.2015.12.058
PubMed: 26768129
Affiliations:
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Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France. 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Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Debeer, Sabine" sort="Debeer, Sabine" uniqKey="Debeer S" first="Sabine" last="Debeer">Sabine Debeer</name><affiliation wicri:level="3"><nlm:affiliation>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Piras, Fabienne" sort="Piras, Fabienne" uniqKey="Piras F" first="Fabienne" last="Piras">Fabienne Piras</name><affiliation wicri:level="3"><nlm:affiliation>Sanofi Pasteur, 1541 avenue Marcel Mérieux, 69280 Marcy L'Etoile, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Sanofi Pasteur, 1541 avenue Marcel Mérieux, 69280 Marcy L'Etoile</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Marcy L'Etoile</settlement></placeName></affiliation></author><author><name sortKey="Andreoni, Christine" sort="Andreoni, Christine" uniqKey="Andreoni C" first="Christine" last="Andréoni">Christine Andréoni</name><affiliation wicri:level="3"><nlm:affiliation>Merial S.A.S., Laboratoire Lyon Gerland, 254 rue Marcel Mérieux, 69007 Lyon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Merial S.A.S., Laboratoire Lyon Gerland, 254 rue Marcel Mérieux, 69007 Lyon</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Boudet, Florence" sort="Boudet, Florence" uniqKey="Boudet F" first="Florence" last="Boudet">Florence Boudet</name><affiliation wicri:level="3"><nlm:affiliation>Sanofi Pasteur, 1541 avenue Marcel Mérieux, 69280 Marcy L'Etoile, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Sanofi Pasteur, 1541 avenue Marcel Mérieux, 69280 Marcy L'Etoile</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Marcy L'Etoile</settlement></placeName></affiliation></author><author><name sortKey="Laurent, Philippe" sort="Laurent, Philippe" uniqKey="Laurent P" first="Philippe" last="Laurent">Philippe Laurent</name><affiliation wicri:level="3"><nlm:affiliation>BD Medical-Pharmaceutical Systems, 11, rue Aristide Bergès, 38800 Le Pont de Claix, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BD Medical-Pharmaceutical Systems, 11, rue Aristide Bergès, 38800 Le Pont de Claix</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Le Pont de Claix</settlement></placeName></affiliation></author><author><name sortKey="Kaiserlian, Dominique" sort="Kaiserlian, Dominique" uniqKey="Kaiserlian D" first="Dominique" last="Kaiserlian">Dominique Kaiserlian</name><affiliation wicri:level="3"><nlm:affiliation>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France. Electronic address: dominique.kaiserlian@inserm.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Dubois, Bertrand" sort="Dubois, Bertrand" uniqKey="Dubois B" first="Bertrand" last="Dubois">Bertrand Dubois</name><affiliation wicri:level="3"><nlm:affiliation>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon, France. Electronic address: bertrand.dubois@lyon.unicancer.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CIRI, International Center for Infectiology Research, "Mucosal immunity, Vaccination & Biotherapies" Team, Université de Lyon, Lyon, France; Inserm, U1111, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France; CNRS, UMR 5308, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="eISSN">1873-2518</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Animals</term><term>Antibodies, Neutralizing (blood)</term><term>Antibodies, Viral (blood)</term><term>Cytokines</term><term>Dendritic Cells (immunology)</term><term>Female</term><term>Immunity, Cellular</term><term>Immunity, Humoral</term><term>Immunity, Innate</term><term>Injections, Intradermal</term><term>Pseudorabies (prevention & control)</term><term>Skin (immunology)</term><term>Swine</term><term>Vaccination (methods)</term><term>Vaccines, Subunit (administration & dosage)</term><term>Vaccines, Subunit (immunology)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Anticorps neutralisants (sang)</term><term>Cellules dendritiques (immunologie)</term><term>Cytokines</term><term>Femelle</term><term>Immunité cellulaire</term><term>Immunité humorale</term><term>Immunité innée</term><term>Injections intradermiques</term><term>Maladie d'Aujeszky ()</term><term>Peau (immunologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Suidae</term><term>Vaccination ()</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins sous-unitaires (administration et posologie)</term><term>Vaccins sous-unitaires (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, Subunit</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Vaccines, Subunit</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins sous-unitaires</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules dendritiques</term><term>Peau</term><term>Protéines de l'enveloppe virale</term><term>Vaccins antiviraux</term><term>Vaccins sous-unitaires</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Dendritic Cells</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Pseudorabies</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Immunity, Cellular</term><term>Immunity, Humoral</term><term>Immunity, Innate</term><term>Injections, Intradermal</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Animaux</term><term>Cytokines</term><term>Femelle</term><term>Immunité cellulaire</term><term>Immunité humorale</term><term>Immunité innée</term><term>Injections intradermiques</term><term>Maladie d'Aujeszky</term><term>Suidae</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Intradermal (ID) vaccination constitutes a promising approach to induce anti-infectious immunity. This route of immunization has mostly been studied with influenza split-virion vaccines. However, the efficacy of ID vaccination for sub-unit vaccines in relation to underlying skin innate immunity remains to be explored for wider application in humans. Relevant animal models that more closely mimic human skin immunity than the widely used mouse models are therefore necessary. Here, we show in domestic swine, which shares striking anatomic and functional properties with human skin, that a single ID delivery of pseudorabies virus (PRV) glycoproteins without added adjuvant is sufficient to trigger adaptive cellular and humoral immune responses, and to confer protection from a lethal respiratory infection with PRV. Analysis of early events at the skin injection site revealed up-regulation of pro-inflammatory cytokine and chemokine genes, recruitment of neutrophils and monocytes and accumulation of inflammatory DC. We further show that the sustained induction of pro-inflammatory cytokine genes results from the combined effects of skin puncture, liquid injection in the dermis and viral antigens. These data highlight that immune protection against respiratory infection can be induced by ID vaccination with a subunit vaccine and reveal that adjuvant requirements are circumvented by the mechanical and antigenic stress caused by ID injection, which triggers innate immunity and mobilization of inflammatory DC at the immunization site. ID vaccination with sub-unit vaccines may thus represent a safe and efficient solution for protection against respiratory infections in swine and possibly also in humans, given the similarity of skin structure and function in both species. </div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Le Pont de Claix</li><li>Lyon</li><li>Marcy L'Etoile</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Le Luduec, Jean Benoit" sort="Le Luduec, Jean Benoit" uniqKey="Le Luduec J" first="Jean-Benoît" last="Le Luduec">Jean-Benoît Le Luduec</name></region><name sortKey="Andreoni, Christine" sort="Andreoni, Christine" uniqKey="Andreoni C" first="Christine" last="Andréoni">Christine Andréoni</name><name sortKey="Boudet, Florence" sort="Boudet, Florence" uniqKey="Boudet F" first="Florence" last="Boudet">Florence Boudet</name><name sortKey="Debeer, Sabine" sort="Debeer, Sabine" uniqKey="Debeer S" first="Sabine" last="Debeer">Sabine Debeer</name><name sortKey="Dubois, Bertrand" sort="Dubois, Bertrand" uniqKey="Dubois B" first="Bertrand" last="Dubois">Bertrand Dubois</name><name sortKey="Kaiserlian, Dominique" sort="Kaiserlian, Dominique" uniqKey="Kaiserlian D" first="Dominique" last="Kaiserlian">Dominique Kaiserlian</name><name sortKey="Laurent, Philippe" sort="Laurent, Philippe" uniqKey="Laurent P" first="Philippe" last="Laurent">Philippe Laurent</name><name sortKey="Piras, Fabienne" sort="Piras, Fabienne" uniqKey="Piras F" first="Fabienne" last="Piras">Fabienne Piras</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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